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Cationic polymer-coated nanoparticles for gene therapy.
The benefits of nonviral gene therapy are enormous, and as the primary limitations to its use are the lack of efficient uptake and the lack of targeting, research on new strategies for improving the efficiency of gene delivery is ongoing. DNA condensed with the cationic polymers polylysine (Pl) and polyethyleneimine (PEI) has been used in gene therapy for a long time, but such methods have been rendered mostly ineffective due to the limited biological stability of the condensing agents, their limited ability to cross the cell membrane, and/or their cytotoxicity. In this paper, a novel approach to improving the efficacy of gene delivery by coupling a polymeric coating to a DNA condensing agent is presented. Several advantages to this approach, including higher transfection efficiency in various cell lines, and a safer approach are discussed.Q:
Determine (and remove) spam and offensive content
What is the best way to detect and remove spam and offensive content from a site or forum?
I'm looking for a solution that works across the board. I don't just want to remove content from the site, I want to protect the users from themselves. So if a user is saying something that will trigger spam detection then that user shouldn't be able to post that, and if a user is trying to start a flamewar then that user shouldn't be able to do that either.
I know about flagging users and re-assigning users that have been banned, but sometimes a user will accidentally post something offensive and they will just delete it.
I'm looking for something that can be applied to a site/forum by default and apply to all users.
Are there any existing solutions that can do this?
A:
We use SpamAssassin and a big hammer to remove posts with matches. SpamAssassin is well documented, and there are other spam software packages that are even better. And, I am pretty sure that most forums and site administration software packages have built in SpamAssassin filters 01e38acffe
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